5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes

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Title : 5-Hydroxytryptamine decreases somatostatin receptors and somatostatin-responsive adenylyl cyclase in rat pancreatic acinar membranes
Author : Muñoz Acedo, Gema; Rodríguez Martín, Eulalia; Puebla Jiménez, Lilian; Arilla Ferreiro, Eduardo
Publisher : Elsevier
Date : 1997
Filiación : Universidad de Alcalá. Departamento de Bioquímica y Biología Molecular
Cita bibliográfica : Life Sciences, 1997, v. 60, n. 22, p. 1965-1976
Keyword : Somatostatin
Serotonin
Pancreas
Proyecto : 0.01/96 (Universidad de Alcalá); PM95-0041 (Ministerio de Educación y Cultura)
Tipo de documento : info:eu-repo/semantics/article
Enlace permanente (URI) : http://hdl.handle.net/10017/2248
DOI : 10.1016/S0024-3205(97)00161-6
ISSN : 0024-3205
Versión : info:eu-repo/semantics/publishedVersion
Version of the editor : http://www.dx.doi.org/10.1016/S0024-3205(97)00161-6
Derechos : (c) Elsevier, 1997
Derechos de acceso : info:eu-repo/semantics/openAccess

Abstract :

Pretreatment of pancreatic acini with 5-hydroxytryptamine (5-HT) reduced the binding of the labeled somatostatin (SS) analogue 125I-Tyr3-SMS to pancreatic acinar membranes. This effect was dependent of the dose of 5-HT used and length of pretreatment. This inhibitory effect of 5-HT was abolished when pancreatic acini were pretreated with 5-HT in the presence of the 5-HT1P receptor-antagonist 5-hydroxytryptophyl-5-hydroxytryptophan amide (5-HTP-DP). Pretreatment of pancreatic acini with 5-HT reduced the inhibition by the stable SS analogue SMS 201-995 of basal and forskolin (FK)-stimulated adenylyl cyclase (AC) activity in pancreatic acinar membranes. There was no statistical difference established between IC50 values for the stable GTP analogue 5'-guanylylimidodiphosphate (Gpp(NH)p) which inhibits ligand binding to SMS receptors in controls and in 5-HT treated pancreatic cells, respectively. In addition, no significant differences were seen in the level of Gi proteins in the control and 5-HT treated panceatic acini. These data suggest that the decrease of the number of 125I-Tyr3-SMS receptors, would explain the decreased sensitivity of AC to SMS 201-995 in membranes from 5-HT-pretreated acini.


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